Optimized assay for antisperm cell-mediated immunity

Optimized assay for antisperm cell-mediated immunity.

Previous studies on antisperm cell-mediated immunity (CMI) have been confounded by the presence of immunogenic leukocytes in sperm antigen preparations. In this study we isolated pure populations of viable spermatozoa on discontinuous Percoll gradients, and utilized sonicated and cavitated extracts, as well as live motile spermatozoa, to measure cellular immunity to spermatozoa in vasectomized ...

EVALUATION OF CELL-MEDIATED IMMUNITY IN MUSTARD GAS INJURIES

Yamakido, et al. in 1986 have studied immunological parameters in poison gas workers, and a depression has been observed in immunologic response. Also, the frequency of cancer has reached as high as five-fold that of the general population. In the present study, cell-mediated immunity (CMI) was measured in three groups of Iranian mustard gas-injured patients. The first group were those wh...

Validity of a rapid assay for antisperm antibodies in semen.

OBJECTIVE To determine the validity of a rapid assay for antisperm antibodies in semen. DESIGN Prospective comparison of the results of standard and rapid antisperm antibody assays performed simultaneously. SETTING Tertiary care infertility center. PATIENT(S) Couples who presented for infertility evaluation. INTERVENTION(S) Semen analysis and measurement of antisperm antibodies in semen...

Humoral Immunity and Cell-Mediated Immunity

Humoral Immunity and Cell-Mediated Immunity